‘(ii)

cutting or boning, prior to reaching the temperature referred to in point 2(b) of Chapter V of Section I of Annex III to Regulation (EC) No 853/2004, is applied in accordance with point 4 of Chapter V of Section I of Annex III to that Regulation;’;

(a)

in point A.1:

(b)

point A.2 is replaced by the following:

‘2.   Collecting of specimens and quantity to be digested

As stipulated in point 4.2 of the ISO 18743:2015 (see also Annexes A and B thereto for further details).’;

(c)

in point A.3, points III and IV are replaced by the following:

‘III.   Recovery of larvae by sedimentation

Where the digests are unclear, they must be clarified as follows:

IV.   Positive or doubtful results

Where examination of a collective sample produces a positive or uncertain result, a further 20 g sample is taken from each pig, as stipulated in point 4.2 of the ISO 18743:2015 (see also Annexes A and B thereto for further details). The 20 g samples from five pigs are pooled and examined using the method described in this Chapter. In this way samples from 20 groups of five pigs will be examined. When Trichinella is detected in a pooled sample from five pigs, further 20 g samples are collected from the individual pigs in the group and each is examined separately using the method described in this Chapter. Parasite samples are to be kept in 70-90 % (final concentration) ethyl alcohol for conservation and identification at species level at the EU or national reference laboratory. For decontamination procedure, see point 12 of the ISO 18743:2015.’;

(d)

point B.2 is replaced by the following:

‘2.   Collecting of specimens

As stipulated in point 4.2 of the ISO 18743:2015 (see also Annexes A and B thereto for further details).’;

(e)

point B.3 is amended as follows:

(f)

point C.1 is amended as follows:

(g)

point C.2 is replaced by the following:

‘2.   Collecting of specimens

As stipulated in point 4.2 of the ISO 18743:2015 (see also Annexes A and B thereto for further details).’;

(h)

in point C.3, point VI is replaced by the following:

‘VI.   Positive or doubtful results

As laid down in point IV of Section A(3).’;

(i)

point D.1 is amended as follows:

(j)

point D.2 is replaced by the following:

‘2.   Collecting of specimens

As stipulated in point 4.2 of the ISO 18743:2015 (see also its Annexes A and B for further details).’;

(k)

in point D.3, points II and III are replaced by the following:

‘II.   Pools of less than 100 g as set out in in point 8 of the ISO 18743:2015

Where needed, up to 15 g can be added to a total pool of 100 g and examined together with these samples in accordance with Section I. More than 15 g must be examined as a complete pool. For pools of up to 50 g, the digestion fluid and the ingredients may be reduced to 1 litre of water, 8 ml of hydrochloric acid and 5 g of pepsin.

III.   Positive or doubtful results

Where examination of a collective sample produces a positive or uncertain latex agglutination result, a further 20 g sample is taken from each swine in accordance with point 4.2 of the ISO 18743:2015 (see also Annexes A and B thereto for further details). The 20 g samples from five swine are pooled and examined using the method described in Section I. In this way samples from 20 groups of five swine must be examined.

When a positive latex agglutination is obtained from a group of five swine, further 20 g samples are collected from the individuals in the group and each is examined separately using the method described in Section I.

When a positive or uncertain latex agglutination result is obtained, at least 20 g of swine muscle must be sent to the national reference laboratory for confirmation using the ISO 18743:2015 or one of the equivalent methods as described above.

Parasite samples must be kept in 70-90 % (final concentration) ethyl alcohol for conservation and identification at species level at the EU or national reference laboratory.

After parasite collection, positive fluids must be decontaminated by heating to at least 60 °C.’.